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Figure 4: Changes in the expression of G0/G1 phase cell cycle regulatory proteins in the apiole-treated COLO-205 xenograft tumors. As previously described,[19] the frozen tumors were pulverized in liquid N2, mixed with lysis buffer (0.5 M Tris– HCl, pH 6.8, and 0.4% SDS) and analyzed by western blotting. Xenograft tumor tissues were thawed in 750 μL of lysis buffer containing protease inhibitors to examine protein expression.[16] The samples were homogenized three times on ice using a PRO200 homogenizer (PRO Scientifi c Inc., Monroe, CT, USA) at setting 3 (18,000 rpm). Protein (50 μg) from each sample was separated via 12% sodium dodecyl sulfate- polyacrylamide gelelectrophoresis, transferred to a nitrocellulose membrane, and analyzed using western blotting

Figure 4: Changes in the expression of G0/G1 phase cell cycle regulatory proteins in the apiole-treated COLO-205 xenograft tumors. As previously described,[19] the frozen tumors were pulverized in liquid N2, mixed with lysis buffer (0.5 M Tris– HCl, pH 6.8, and 0.4% SDS) and analyzed by western blotting. Xenograft tumor tissues were thawed in 750 μL of lysis buffer containing protease inhibitors to examine protein expression.<sup>[16]</sup> The samples were homogenized three times on ice using a PRO200 homogenizer (PRO Scientifi c Inc., Monroe, CT, USA) at setting 3 (18,000 rpm). Protein (50 μg) from each sample was separated via 12% sodium dodecyl sulfate- polyacrylamide gelelectrophoresis, transferred to a nitrocellulose membrane, and analyzed using western blotting