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Figure 2: SYBR Green real time-telomeric repeat amplification protocol assay was used for evaluating of telomerase activity in MDCK cell lysates. The fluorescence signals of SYBR Green were shown on the y-axis, against the cycle number on the x-axis. The values of the threshold cycle were 24.69 and 27.78 in nontransfected and transfected cells, respectively. The fluorescence amount detected for the first time at the top of the baseline is considered as the cycle threshold (ct)

Figure 2: SYBR Green real time-telomeric repeat amplification protocol assay was used for evaluating of telomerase activity in MDCK cell lysates. The fluorescence signals of SYBR Green were shown on the y-axis, against the cycle number on the x-axis. The values of the threshold cycle were 24.69 and 27.78 in nontransfected and transfected cells, respectively. The fluorescence amount detected for the  first time at the top of the baseline is considered as the cycle threshold (ct)