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Figure 4: The PNA clamping system. The PNA oligomer was designed to bind to the bottom strand of the wild-type sequence, spanning mutational hotspots in exons 18–21 of the epidermal growth factor receptor gene. The forward PCR primer partially overlapped the PNA-binding site. (a) A PNA/DNA hybrid with a perfect match prevents annealing of the PCR primer and amplification of wild-type DNA. (b) A PNA/DNA hybrid with a single base pair mismatch does not suppress annealing of the PCR primer or amplification of mutant alleles (J Thorac Oncol 2012;7:355-64). PNA = Peptide nucleic acid, PCR = Polymerase chain reaction

Figure 4: The PNA clamping system. The PNA oligomer was designed to bind to the bottom strand of the wild-type sequence, spanning mutational hotspots in exons 18–21 of the epidermal growth factor receptor gene. The forward PCR primer partially overlapped the PNA-binding site. (a) A PNA/DNA hybrid with a perfect match prevents annealing of the PCR primer and amplification of wild-type DNA. (b) A PNA/DNA hybrid with a single base pair mismatch does not suppress annealing of the PCR primer or amplification of mutant alleles (J Thorac Oncol 2012;7:355-64). PNA = Peptide nucleic acid, PCR = Polymerase chain reaction