Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 15  |  Issue : 4  |  Page : 927--932

Interleukin 10 promotes growth and invasion of glioma cells by up-regulating KPNA 2 in vitro


Zihao Zhang1, Xiaoming Huang2, Jie Li2, Haitao Fan2, Fan Yang2, Rui Zhang2, Yihang Yang2, Shaobin Feng2, Dong He2, Wei Sun3, Tao Xin4 
1 Department of Medical, Queen Mary School, Nanchang University, Nanchang, Jiangxi, China
2 Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China
3 Department of Neurosurgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, China
4 Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong; Department of Neurosurgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, China

Correspondence Address:
Wei Sun
No. 92 Aiguo Road, Nanchang, 330006 Jiangxi
China

Objective: Glioma is one of the leading causes of death worldwide with high incidence, recurrence, and mortality. Interleukin-10 (IL-10) is a cytokine with dual function in many types of tumors. Although IL-10 is overexpressed and promotes tumor progression in human primary brain tumor, the mechanisms are largely unknown. Materials and Methods: Glioma cells were treated with different dosages of IL-10. The cell growth was detected by CCK-8, and the invasion was measured by Transwell. The relative expression of messenger RNAs was detected by quantitative real-time polymerase chain reaction. Results: We found that IL-10 treatment significantly enhanced glioma cell growth and invasion. Moreover, KPNA2 was significantly upregulated after treatment with IL-10. By performing knockdown experiments, we found that the glioma cell growth and invasion were significantly declined. Conclusions: The results indicated that knockdown of KPNA2 significantly inhibited the growth and invasion of glioma cells. Moreover, IL-10 promotes glioma progression via upregulation of KPNA2. This study will be of important significance and provides a potential target for treatment of patients with glioma.


How to cite this article:
Zhang Z, Huang X, Li J, Fan H, Yang F, Zhang R, Yang Y, Feng S, He D, Sun W, Xin T. Interleukin 10 promotes growth and invasion of glioma cells by up-regulating KPNA 2 in vitro.J Can Res Ther 2019;15:927-932


How to cite this URL:
Zhang Z, Huang X, Li J, Fan H, Yang F, Zhang R, Yang Y, Feng S, He D, Sun W, Xin T. Interleukin 10 promotes growth and invasion of glioma cells by up-regulating KPNA 2 in vitro. J Can Res Ther [serial online] 2019 [cited 2020 Apr 4 ];15:927-932
Available from: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2019;volume=15;issue=4;spage=927;epage=932;aulast=Zhang;type=0