Year : 2018 | Volume
: 14 | Issue : 8 | Page : 41--45
Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation
Ping Zhan, Ling Liu, Dan Nie, Yaofang Liu, Xiguang Mao
Department of Gynecology, The Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan, China
Purpose: To explore the influence of intermediate-conductance-Ca2+-activated K+ channels. (IKCal) on HeLa cell proliferation.
Materials and Methods: An IKCal blocking agent (clotrimazole (CLT)) and small hairpin ribonucleic acid interference (RNAi) was used to block IKCal in HeLa cells; subsequently, cell growth was observed. Furthermore, the messenger ribonucleic acid (mRNA) expression of IKCal was detected by reverse transcriptase polymerase chain reaction (RT-PCR) after IKCal-blocking.
Results: The obvious morphological changes in HeLa cells were observed 48 h after CLT-blocking. The PCR results indicated that CLT reduced the mRNA expression of IKCal in HeLa cells. HeLa cells were transfected with pGenesil via RNAi; the HeLa cells transfected with pGenesil-IK displayed obvious morphological changes 48 h after transfection. In addition, RT-PCR further demonstrated the reduced mRNA expression of IKCal in the pGenesil group.
Conclusion: CLT and blocking of IKCal gene expression effectively inhibits HeLa cell proliferation; therefore, the use of a blocking agent and RNAi both effectively downregulated the mRNA expression of IKCal, which in turn mediated the proliferation of HeLa cells, producing an antitumor effect.
Department of Gynecology, The Affiliated Hospital of Luzhou Medical College, No. 25 Taiping, Luzhou-646 000, Sichuan
|How to cite this article:|
Zhan P, Liu L, Nie D, Liu Y, Mao X. Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation.J Can Res Ther 2018;14:41-45
|How to cite this URL:|
Zhan P, Liu L, Nie D, Liu Y, Mao X. Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation. J Can Res Ther [serial online] 2018 [cited 2019 Oct 23 ];14:41-45
Available from: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2018;volume=14;issue=8;spage=41;epage=45;aulast=Zhan;type=0