Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 8  |  Page : 41--45

Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation


Ping Zhan, Ling Liu, Dan Nie, Yaofang Liu, Xiguang Mao 
 Department of Gynecology, The Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan, China

Correspondence Address:
Xiguang Mao
Department of Gynecology, The Affiliated Hospital of Luzhou Medical College, No. 25 Taiping, Luzhou-646 000, Sichuan
China

Purpose: To explore the influence of intermediate-conductance-Ca2+-activated K+ channels. (IKCal) on HeLa cell proliferation. Materials and Methods: An IKCal blocking agent (clotrimazole (CLT)) and small hairpin ribonucleic acid interference (RNAi) was used to block IKCal in HeLa cells; subsequently, cell growth was observed. Furthermore, the messenger ribonucleic acid (mRNA) expression of IKCal was detected by reverse transcriptase polymerase chain reaction (RT-PCR) after IKCal-blocking. Results: The obvious morphological changes in HeLa cells were observed 48 h after CLT-blocking. The PCR results indicated that CLT reduced the mRNA expression of IKCal in HeLa cells. HeLa cells were transfected with pGenesil via RNAi; the HeLa cells transfected with pGenesil-IK displayed obvious morphological changes 48 h after transfection. In addition, RT-PCR further demonstrated the reduced mRNA expression of IKCal in the pGenesil group. Conclusion: CLT and blocking of IKCal gene expression effectively inhibits HeLa cell proliferation; therefore, the use of a blocking agent and RNAi both effectively downregulated the mRNA expression of IKCal, which in turn mediated the proliferation of HeLa cells, producing an antitumor effect.


How to cite this article:
Zhan P, Liu L, Nie D, Liu Y, Mao X. Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation.J Can Res Ther 2018;14:41-45


How to cite this URL:
Zhan P, Liu L, Nie D, Liu Y, Mao X. Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation. J Can Res Ther [serial online] 2018 [cited 2019 Oct 23 ];14:41-45
Available from: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2018;volume=14;issue=8;spage=41;epage=45;aulast=Zhan;type=0