Journal of Cancer Research and Therapeutics

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 14  |  Issue : 12  |  Page : 1070--1075

Radioprotective effect of melatonin on expression of Cdkn1a and Rad50 genes in rat peripheral blood


Hamed Rezaeejam1, Alireza Shirazi1, Pantea Izadi2, Javad Tavakkoly Bazzaz2, Mahmoud Ghazi-Khansari3, Majid Valizadeh4, Ghasem Azizi Tabesh2 
1 Department of Medical Physics and Biomedical Engineering, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4 Department of Medical Physics, Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran, Iran

Correspondence Address:
Pantea Izadi
Department of Medical Genetics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran
Iran

Objective: Ionizing radiation is a critical threat to biomolecules, especially DNA. Various combinatorial compounds have been studied to protect this biomolecule. Melatonin has been reported as a direct and indirect free radical scavenger, but in this study, we explored the effect of melatonin on assisting in DNA repair by expression of Cdkn1a and Rad50; both of these genes are involved in DNA repair signaling, induced by radiation in rat peripheral blood. Materials and Methods: Rats were irradiated with single whole-body linear accelerator X-ray radiation doses of 2 and 8 Gy with or without melatonin (100 mg/kg body weight) pretreatments. The rats were randomly divided into nine groups and given an intraperitoneal injection of melatonin or the same volume of vehicle alone 1 h before radiation. Blood samples were taken 8, 24, and 48 h postradiation to measure gene expression of Cdkn1a and Rad50 using quantitative reverse transcription polymerase chain reaction technique. Results: Melatonin pretreatment increased the expression of Cdkn1a and Rad50 in 8 and 24 h postradiations (2 and 8 Gy) (P < 0.05), and there was no significant difference in 48 h postradiation compared to the radiation-only and vehicle plus radiation (2 and 8 Gy) groups. Conclusions: Based on our results, pretreatment with melatonin (100 mg/kg) may ameliorates injurious effects of 2 and 8 Gy ionization radiation by increasing the expression level of Cdkn1a and Rad50 in rat peripheral blood and assist in DNA double-strand breaks repair, especially during the early postradiation.


How to cite this article:
Rezaeejam H, Shirazi A, Izadi P, Bazzaz JT, Ghazi-Khansari M, Valizadeh M, Tabesh GA. Radioprotective effect of melatonin on expression of Cdkn1a and Rad50 genes in rat peripheral blood.J Can Res Ther 2018;14:1070-1075


How to cite this URL:
Rezaeejam H, Shirazi A, Izadi P, Bazzaz JT, Ghazi-Khansari M, Valizadeh M, Tabesh GA. Radioprotective effect of melatonin on expression of Cdkn1a and Rad50 genes in rat peripheral blood. J Can Res Ther [serial online] 2018 [cited 2019 Oct 18 ];14:1070-1075
Available from: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2018;volume=14;issue=12;spage=1070;epage=1075;aulast=Rezaeejam;type=0