Year : 2014 | Volume
: 10 | Issue : 3 | Page : 531--534
Lack of human papillomavirus DNA in colon adenocarcinama and adenoma
Arezoo Aghakhani1, Rasool Hamkar2, Amitis Ramezani1, Farahnaz Bidari-Zerehpoosh3, Shahram Sabeti3, Nastaran Ghavami2, Mohammad Banifazl4, Niloofar Rashidi1, Ali Eslamifar1
1 Department of Clinical Research, Pasteur Institute of Iran, Tehran, Iran
2 Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3 Pathology Ward, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Iranian Society for Support of Patients with Infectious Diseases, Tehran, Iran
Background: Human papilloma viruses (HPV) have been detected in several types of cancers. Over the last few years, a possible correlation between HPV infection and colon cancers has been suggested. The aim of this study was to assess the presence of HPV-DNA in colon adenocarcinomas and adenomas to describe the relationship of HPV infection with these pathologic features.
Materials and Methods: The study included formalin-fixed and paraffin-embedded tissue samples of 70 patients with colon adenocarcinoma and 30 patients with adenoma (as study group) and 30 tumor adjacent tissues (as control). HPV-DNA was purified and first amplified through MY09/MY11 and GP5+/GP6+ primers. Subsequently, for more precision, another PCR was performed using PGMY09/11 L1 consensus primer system.
Results: All tested adenocarcinomas and adenomas as well as normal tumor adjacent tissues were negative for all types of HPV in two PCR assays.
Conclusion: Our results do not support the relationship between HPV infection and colon carcinoma or adenoma. Attributing a role to the HPV in the etiology of colon carcinogenesis will require further studies.
Department of Clinical Research, Pasteur Institute of Iran,No 69, Pasteur Ave., Tehran, 13164
|How to cite this article:|
Aghakhani A, Hamkar R, Ramezani A, Bidari-Zerehpoosh F, Sabeti S, Ghavami N, Banifazl M, Rashidi N, Eslamifar A. Lack of human papillomavirus DNA in colon adenocarcinama and adenoma.J Can Res Ther 2014;10:531-534
|How to cite this URL:|
Aghakhani A, Hamkar R, Ramezani A, Bidari-Zerehpoosh F, Sabeti S, Ghavami N, Banifazl M, Rashidi N, Eslamifar A. Lack of human papillomavirus DNA in colon adenocarcinama and adenoma. J Can Res Ther [serial online] 2014 [cited 2020 Apr 7 ];10:531-534
Available from: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2014;volume=10;issue=3;spage=531;epage=534;aulast=Aghakhani;type=0