Year : 2013 | Volume
: 9 | Issue : 1 | Page : 22--24
A preliminary study on the radiation-resistance mechanism in ovarian cancer
Qi Liao1, Hong-mei Zhang2, Hai-hua Li4, Ren Zhou4, Hui-ling Mao4, Yu-bing Chen3, Man-Hua Cui4
1 Department of Gynaecology and Obstetrics, Second Teaching Hospital of Jilin University,Changchun; Department of Gynaecology and Obstetrics, The Da Qing Long Nan Hospital, Daqing, HeiLong Jing, China
2 Laboratory of TANG Ao-qing Distinguished Professor, China-Japan Union Hospital of Jilin University, Changchun, P. R, China
3 Department of Radiotherapy, Second Teaching Hospital of Jilin University, Changchun, China
4 Department of Gynaecology and Obstetrics, Second Teaching Hospital of Jilin University, Changchun, China
Aim: The present study was designed to explore the radiation-resistance mechanism by interfering in checkpoints kinase 1 (CHK1) and DNA-activated protein kinase (DNA-PK) genes with short hairpin RNA (shRNA) transfection into Skov3 cells derived from ovarian cancer and HeLa cells derived from cervical cancer.
Materials and Methods: The cultured Skov3 and HeLa cells were transfected with plasmid vectors containing CHK1 shRNA and DNA-PK shRNA, respectively, through Lipofectimine™ 2000 mediation, and cultured for 20 hours before exposure to 2 Gy X-radiation. The cells were harvested 4 and 28 after X-irradiation respectively then washed 3 times with PBS. These cells were stained with Annexin V/PI and applied by flow cytometer to analyze alteration of apoptosis with software CellQuest.
Results: The apoptotic response in Skov3 cells to X-radiation was significantly lower than that in HeLa cells at 4 hour (t = 15.22, P < 0.001) and 28 hours (t = 15.78, P < 0.001) of post-irradiation. The shRNA might not affect the apoptosis of Skov3 and HeLa cells, while shRNA-transfection significantly enhanced the apoptotic response in Skov3 cells to X-radiation as compared with that in HeLa cells.
Conclusions: The present work suggests that the CHK1 and DNA-PK genes are very likely to play a role in developing a radiation resistance in ovarian cancer.
Second Clinical Hospital of Jilin University, 218 Ziqiang street, Changchun 130041, P. R
|How to cite this article:|
Liao Q, Zhang Hm, Li Hh, Zhou R, Mao Hl, Chen Yb, Cui MH. A preliminary study on the radiation-resistance mechanism in ovarian cancer.J Can Res Ther 2013;9:22-24
|How to cite this URL:|
Liao Q, Zhang Hm, Li Hh, Zhou R, Mao Hl, Chen Yb, Cui MH. A preliminary study on the radiation-resistance mechanism in ovarian cancer. J Can Res Ther [serial online] 2013 [cited 2020 Jul 7 ];9:22-24
Available from: http://www.cancerjournal.net/article.asp?issn=0973-1482;year=2013;volume=9;issue=1;spage=22;epage=24;aulast=Liao;type=0