Nueclear Web Banner
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
 
ORIGINAL ARTICLE
Ahead of Print

Establishment of a drug-resistant human cervical cancer cell line and research on its drug-resistance


1 Department of Gynecology, Zhujiang Hospital of Southern Medical University; Department of Gynecology, Bao'an District Maternity and Child Health Hospital of Shenzhen, Shenzhen, Guangdong, China
2 Department of Gynecology, Bao'an District Maternity and Child Health Hospital of Shenzhen, Shenzhen, Guangdong, China
3 Department of Gynecology, Zhujiang Hospital of Southern Medical University, Guangzhou, China

Correspondence Address:
Yifeng Wang,
Department of Gynecology, Zhujiang Hospital of Southern Medical University, No. 253 Industrial Avenue, Guangzhou, Guangdong
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None

Aims: To investigate the differences and international connections between the human cervical cancer cell line (HeLa cells) and the Taxol-resistant HeLa cell line (HeLa/Taxol). Materials and Methods: As parental cells, HeLa cells were cultured in stepwise escalating concentration of Taxol from 0.01 µg/ml (11.7 × 10−9 mol/L) to 0.5 µg/ml (585 × 10−9 mol/L). The drug resistance of HeLa/Taxol cells was detected by methyl-thiazolyl-tetrazolium assay. Real time-polymerase chain reaction (RT-PCR) was conducted to detect the messenger RNA levels of drug resistance genes and apoptosis-related genes. The proteins levels were detected through immunofluorescence and Western blot. Results: Compared with parental HeLa cells, HeLa/Taxol with Taxol resistance had the following biological characteristics:first, they had a lower growth velocity; second, the expression of P-glycoprotein and glutathione S-transferases was significantly increased; Third, the expression of antiapoptotic protein Bcl-2 and apoptosis inhibitor protein survivin was prominently increased. Conclusion: The drug-resistance in HeLa/Taxol is mainly associated with the high expression of multidrug resistance genes, antiapoptotic protein Bcl-2, and apoptosis inhibitor protein survivin as an important reason for the failure of chemotherapy of tumor tissue.


Print this article
Search
 Back
 
  Search Pubmed for
 
    -  Wang H
    -  Zhu Y
    -  Hao C
    -  Fan J
    -  Liu Y
    -  Wang Y
 Citation Manager
 Article Access Statistics
 Reader Comments
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed255    
    PDF Downloaded13    

Recommend this journal