Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 

 
ORIGINAL ARTICLE
Ahead of print publication  

Anti-tumor effects of phenolic alkaloids of menispermum dauricum on gastric cancer in vivo and in vitro


1 Department of Gastrointestinal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province, China
2 Department of Pharmacology, School Basic Medical Sciences, Heilongjiang University of Chinese Medicine, Harbin 150040, Heilongjiang Province, China

Date of Web Publication28-Jul-2016

Correspondence Address:
Hongfeng Zhang,
Department of Gastrointestinal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin 150040, Heilongjiang Province
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None

 > Abstract 


Aim: This study was conducted to investigate the anti-tumor effects of the Chinese traditional herb phenolic alkaloids of menispermum dauricum (PAMD) on gastric cancer both in vitro and in vivo.
Materials and Methods: Cell apoptosis was detected in cultured SGC-7901 cells after administration of a different dose of PAMD. Gastric cancer model was established by single i.p. injection of SGC-7901 cells in the mice (n = 60). Then, animals were received high dose (20 mg/kg), medial dose (10 mg/kg), and low dose (5 mg/kg) of PAMD. Mice received 5-floxuridine was set as positive controls and received normal saline was as blank controls. Effects of PAMD on tumor growth were evaluated by tumor inhibition rate. Tumor tissues were collected from mice and detected for the expression of several genes P53, B-cell CLL/lymphoma 2 (BCL-2), BCL-2-associated X protein (BAX), CASPASE-3, K-RAS by real-time polymerase chain reaction, and Western blot. In addition, tumor cell changes were observed under transmission electron microscopy.
Results: The apoptosis index in PAMD at high- and medial-dose group was significantly higher than that in blank control group (P < 0.01). PAMD at different dose could significantly decrease the tumor weight compared to the blank control group (P < 0.01). In addition, PAMD could obviously increase BAX and caspase-3 expression as well as decrease K-RAS expression when compared to the blank control treatment (P < 0.01). Furthermore, PAMD could induce tumor cell morphology changes.
Conclusions: PAMD could suppress gastric tumor growth in vivo, possibly through increasing the expression of pro-apoptotic genes expression then leading to cell apoptosis and inhibiting oncogenic K-RAS expression.

Keywords: Cell apoptosis, gastric cancer, in vivo, K-RAS, phenolic alkaloids of menispermum dauricum



How to cite this URL:
Wu D, Du J, Zhang Y, Su Y, Zhang H. Anti-tumor effects of phenolic alkaloids of menispermum dauricum on gastric cancer in vivo and in vitro. J Can Res Ther [Epub ahead of print] [cited 2017 Jul 26]. Available from: http://www.cancerjournal.net/preprintarticle.asp?id=184521

.




 

 
Top
 
 
  Search
 
     Search Pubmed for
 
    -  Wu D
    -  Du J
    -  Zhang Y
    -  Su Y
    -  Zhang H
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  >Abstract
  In this article

 Article Access Statistics
    Viewed391    
    PDF Downloaded21    

Recommend this journal