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Cigarette smoke condensate could promote human bronchial epithelial BEAS-2B cell migration through shifting neprilysin trafficking

1 Department of Respiratory Medicine, The Third Hospital of Shijiazhuang City, Shijiazhuang 050011, China
2 Department of Anesthesiology, Shandong Cancer Hospital, Shandong Province, China

Correspondence Address:
Kun Yang,
Department of Respiratory Medicine, The Third Hospital of Shijiazhuang City, Shijiazhuang 050011
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Source of Support: None, Conflict of Interest: None

Aim of Study: Recent studies have suggested neprilysin (NEP) play a key role in cigarette smoke-induced nonsmall-cell lung carcinoma; however, the detailed mechanism was still unclear. Here, we employed in vitro human bronchial epithelial BEAS-2B cells to investigate whether and how NEP involved in cigarette smoke condensate (CSC)-induced cancer occurrence. Materials and Methods: In vitro MTT and transwell assay was applied. Live cell imaging and staining were also employed. Results: In vitro data showed that CSC could increase BEAS-2B cell migration while NEP shRNA could block CSC-induced BEAS-2B cell hypermigration. By biotination and live cell staining, we found that after CSC treatment, cell surface NEP was increased while internalization trafficking was shifted from late endosome/lysosome pathway to recycling pathway. Finally, we found that surface NEP could bind to p120 catenin (p120ctn) for lysosome destination turnover while CSC treatment could change p120ctn membrane/cytosome distribution. Loss of p120ctn will subsequently change NEP trafficking and finally, increase its membrane distribution with a phenocopy manner as CSC. Conclusion: These data indicated under CSC treatment; losing of membrane p120ctn could upregulate surface NEP protein level and thus facilitate BEAS-2B cell migration.

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