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Effect of inhibition of intermediate-conductance-Ca2+-activated K+ channels on HeLa cell proliferation

 Department of Gynecology, The Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan, China

Correspondence Address:
Xiguang Mao,
Department of Gynecology, The Affiliated Hospital of Luzhou Medical College, No. 25 Taiping, Luzhou-646 000, Sichuan
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Source of Support: None, Conflict of Interest: None

Purpose: To explore the influence of intermediate.conductance-Ca2+-activated K+ channels. (IKCal) on HeLa cell proliferation. Materials and Methods: An IKCal blocking agent (clotrimazole (CLT)) and small hairpin ribonucleic acid interference (RNAi) was used to block IKCal in HeLa cells; subsequently, cell growth was observed. Furthermore, the messenger ribonucleic acid (mRNA) expression of IKCal was detected by reverse transcriptase polymerase chain reaction (RT-PCR) after IKCal-blocking. Results: The obvious morphological changes in HeLa cells were observed 48 h after CLT.blocking. The PCR results indicated that CLT reduced the mRNA expression of IKCal in HeLa cells. HeLa cells were transfected with pGenesil via RNAi; the HeLa cells transfected with pGenesil.IK displayed obvious morphological changes 48 h after transfection. In addition, RT.PCR further demonstrated the reduced mRNA expression of IKCal in the pGenesil group. Conclusion: CLT and blocking of IKCal gene expression effectively inhibits HeLa cell proliferation; therefore, the use of a blocking agent and RNAi both effectively downregulated the mRNA expression of IKCal, which in turn mediated the proliferation of HeLa cells, producing an antitumor effect.

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