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Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids


1 Department of Surgery, Cheng Ching Hospital, Chung-Kang Branch, Hsinchu, Taiwan
2 Department of Colorectal Surgery, China Medical University Hospital, Taichung, Taiwan
3 Department of Biotechnology and Pharmaceutical Technology, Yuanpei University of Medical Technology, Hsinchu, Taiwan
4 Department of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology, Hsinchu, Taiwan
5 Department of Medical Imaging and Radiological Technology, Yuanpei University of Medical Technology, Hsinchu, Taiwan
6 Department of Biomedical Engineering, Yuanpei University of Medical Technology, Hsinchu, Taiwan

Correspondence Address:
Chih-Ping Hsu,
Department of Medical Laboratory Science and Biotechnology, Yuanpei University of Medical Technology, No. 306 Yuanpei Street, Hsinchu City 30015
Taiwan
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Source of Support: None, Conflict of Interest: None

Aim of Study: Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to inhibit the proliferation and anchorage-independent growth in soft agar of two colorectal carcinoma (CRC) cells in vitro. In this report, we further examined the effects of LFP in a CRC spheroid model. Materials and Methods: A liquid-overlay assay employing HT-29 spheroids was used to evaluate the effects of LFP on cancer cell tumorigenesis, viability, and apoptosis. Associated effects on signaling path ways (epidermal growth factor receptor [EGFR], Akt) and apoptotic regulators were measured using Western blot. Results: Treatment with LFP up to 200 µg/ml inhibited tumor growth in a dose-dependent manner and induced prominent apoptosis as measured by annexin V staining. Cells treated with LFP showed decreased EGFR and Akt phosphorylation with decreased expression of B-cell lymphoma 2. Conclusion: The ability of LFP to induce apoptosis in CRC spheroids warrants further investigation of its composition and identification of tumor-active components.


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