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MicroRNA-21 in peripheral blood mononuclear cells as a novel biomarker in the diagnosis and prognosis of prostate cancer


1 Department of Urology, Shandong Provincial Hospital, Shandong University, Jinan 250021, China
2 Department of Urology, Shougang Hospital of Peking University, Beijing, 100144, China

Correspondence Address:
Kejia Ding,
Department of Urology, Shandong Provincial Hospital, Shandong University, No. 324 Jingwuweiqi Road, Jinan 250021
China
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Source of Support: None, Conflict of Interest: None

Objective: To evaluate the effects of microRNA-21 (miR-21) in the peripheral blood mononuclear cell (PBMC) in the diagnosis and prognosis of prostate cancer (PCa). Materials and Methods: Proved by pathologic biopsy, 92 patients diagnosed with PCa and also underwent resection operation and 85 patients with benign prostatic hyperplasia (BPH) were selected in this study, as well as 97 healthy volunteers were chosen as the control group. PBMC were extracted to examine the relative expression of miR-21 by real-time reverse transcriptase-polymerase chain reaction. The relative operating characteristic (ROC) curves were drawn to analyze the diagnosis value of PCa. The survival function curves were made by Kaplan-Meier method to show the miR-21 expression levels in PCa patients. The log-rank test was adopted to compare the differences among the different groups. The Cox proportional hazard risk regression analysis was used to screen the independent factors affected the PCa patients. Results: The expression levels of miR-21 in PCa group were increased compared to BPH and control group (P < 0.05). ROC curves of PCa and BPH distinguished by the miR-21 demonstrated that the sensitivity and specificity were 80.4% and 90.6%. ROC curves of PCa and the control group distinguished by the miR-21 illustrated that the sensitivity and specificity were 78.3% and 93.8%. The results of Kaplan-Meier Method revealed that the median survival time in low expression of miR-21 were greater than that of in high expression, and the miR-21 expression levels were related to the prognosis of PCa (P < 0.05). The results of the Cox analysis suggested that the miR-21 expression level and bone metastasis could be the independent factors affected the prognosis of PCa patients. Conclusion: miR-21 is highly expressed in the PCa patients, which could be the molecule biomarker of diagnostic and prognosis of PCa.


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