Immunohistochemical evaluation of prime molecules in cervical lesions towards assessment of malignant potentiality
Lopamudra Das1, Sukla Naskar2, Tandra Sarkar3, Ashok Kumar Maiti4, Soumen Das5, Jyotirmoy Chatterjee5
1 Department of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal; Department of Materials Engineering, Indian Institute of Science, Bangalore, Karnataka, India
2 Department of Pathology, Calcutta National Medical College, Kolkata, West Bengal, India
3 Department of Radiology, Apollo Gleanengles Hospital, Kolkata, West Bengal, India
4 Department of Pathology, Midnapore Medical College and Hospital, Midnapore, West Bengal, India
5 Department of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India
Department of Materials Engineering, Biomaterials and Tissue Engineering Lab, Indian Institute of Science, Bangalore - 560 012, Karnataka
Source of Support: None, Conflict of Interest: None
Objective: A comparative immunohistochemical evaluation of p63, CD105, and E-cadherin expression pattern in histopathologically confirmed normal cervical epithelium (NCM), dysplastic cervical epithelium (DYS) and squamous cell carcinoma (SCC) of uterine cervix towards assessing malignant potentiality of the precancerous condition.
Materials and Methods: The biopsies from cervical mucosa (normal, dysplasia, and cancer) were studied by routine hematoxylin and eosin (H and E) and by immunohistochemistry for p63, E-cadherin, and CD105 expression. The expressions of these molecules were assessed in a semiquantitative way by (i) counting p63 cell population and distribution, (ii) intensity scoring of E-cadherin along the expression path, and (iii) measuring CD105 expression density.
Result: p63 + cells were highest in carcinomas followed by dysplasia and normal. An abrupt increase in CD105 expression was observed through change of normal to dysplasia and cancer. A decrease in membranous E-cadherin expression was noticed in the transformation from normal to precancer and cancers.
Conclusion: The malignant potential of the dysplastic conditions is likely to be correlated with upregulation in p63 and CD105 expression and a simultaneous downregulation of membranous E-cadherin.