Luteolin-loading of Her-2-poly (lactic-co-glycolic acid) nanoparticles and proliferative inhibition of gastric cancer cells via targeted regulation of forkhead box protein O1
Jian Ding1, Qiu Li1, ShanShan He1, Jie Xie2, XiaoFei Liang3, Ting Wu1, Dan Li4
1 Digestive Department, The First Affiliated Hospital of Fujian Medical University, Taijiang, P.R. China
2 Digestive Department, Hospital of Fujian Normal University, Fujian Normal University, Shangsan, Cangshan, P.R. China
3 State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China
4 Digestive Department, Union Hospital of Fujian Medical University, Gulou, Fuzhou, Fujian, P.R. China
Department of Gastroenterology, Union Hospital, Fujian Medical University, 29 Xinquan Road, Fuzhou 350001, Fujian Province
Department of Gastroenterology, First Affiliated Hospital, Fujian Medical University, 20 Chazhong Road, Fuzhou 350005, Fujian Province
Source of Support: None, Conflict of Interest: None
Background: Developing the natural medicine that allow for the specific targeting cytotoxicity is a very important research area in the development of anti-tumor drugs.
Aims and Objectives: This study was conducted to determine the targeted inhibitory effects of luteolin-loaded Her-2-poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) on gastric cancer cells and to delineate the mechanism underlying the inhibition of tumors by luteolin.
Materials and Methods: Luteolin-loaded Her-2-PLGA NPs (Her-2-NPs) were prepared, physically and chemically characterized, and their effects on gastric cancer cells were investigated. The rate of NP uptake by cells and the cell morphology were observed using confocal microscopy; the rates of cell proliferation and apoptosis were identified using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide assay and flow cytometry, respectively; and the mRNA and protein expression levels of forkhead box protein O1 (FOXO1) were determined using quantitative polymerase chain reaction and Western blotting, respectively.
Results: Compared with nontargeted microspheres, Her-2-NPs led to significantly enhanced uptake of luteolin by SGC-7901 cells. Luteolin-loaded Her-2-NPs also significantly inhibited the proliferation of gastric cancer cells, weakened their migratory ability, and increased both the mRNA and protein expression levels of FOXO1.
Conclusion: Luteolin-loading of Her-2-NPs could potentially be used as a novel anti-cancer drugs for targeted cancer therapy.