Interleukin 10 promotes growth and invasion of glioma cells by up-regulating KPNA 2 in vitro
Zihao Zhang1, Xiaoming Huang2, Jie Li2, Haitao Fan2, Fan Yang2, Rui Zhang2, Yihang Yang2, Shaobin Feng2, Dong He2, Wei Sun3, Tao Xin4
1 Department of Medical, Queen Mary School, Nanchang University, Nanchang, Jiangxi, China
2 Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, China
3 Department of Neurosurgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, China
4 Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong; Department of Neurosurgery, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi, China
No. 92 Aiguo Road, Nanchang, 330006 Jiangxi
Source of Support: None, Conflict of Interest: None
Objective: Glioma is one of the leading causes of death worldwide with high incidence, recurrence, and mortality. Interleukin-10 (IL-10) is a cytokine with dual function in many types of tumors. Although IL-10 is overexpressed and promotes tumor progression in human primary brain tumor, the mechanisms are largely unknown.
Materials and Methods: Glioma cells were treated with different dosages of IL-10. The cell growth was detected by CCK-8, and the invasion was measured by Transwell. The relative expression of messenger RNAs was detected by quantitative real-time polymerase chain reaction.
Results: We found that IL-10 treatment significantly enhanced glioma cell growth and invasion. Moreover, KPNA2 was significantly upregulated after treatment with IL-10. By performing knockdown experiments, we found that the glioma cell growth and invasion were significantly declined.
Conclusions: The results indicated that knockdown of KPNA2 significantly inhibited the growth and invasion of glioma cells. Moreover, IL-10 promotes glioma progression via upregulation of KPNA2. This study will be of important significance and provides a potential target for treatment of patients with glioma.