Chromatin remodeling factor lymphoid-specific helicase links with Epstein-Barr virus associated the follicular germinal center B cell lymphomas
Chunhui Ouyang1, Zhenghao Deng2, Jianhua Zhou2, Chunyan Fu2, Shuang Liu3, Yongguang Tao4, Desheng Xiao2
1 Department of Gastroenterology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China
2 Department of Pathology, School of Basic Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China
3 Center for Medicine Research, Xiangya Hospital, Central South University, Changsha, Hunan, China
4 Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, School of Basic Medicine, Cancer Research Institute, Central South University, Changsha, Hunan, China
Dr. Desheng Xiao
Department of Pathology, Xiangya Hospital; Department of Pathology, School of Basic Medicine, Central South University, Changsha 410078, Hunan
Source of Support: None, Conflict of Interest: None
Background: We assessed the frequency of epigenetic lesions, including lymphoid-specific helicase (LSH), 5-hydroxymethylcytosine (5-hmC) and E2F1, and the possible correlations among molecular findings, phenotype, clinical features, and outcome.
Methods: We investigated 181 paraffin-embedded B-cell lymphoma samples using immunohistochemistry and in situ hybridization.
Results: The levels of Ki67, LSH, 5-hmC, and E2F1 were all increased in germinal center B-cell lymphomas when compared with those in normal lymph nodes, and LSH was highly expressed in diffuse large B-cell lymphomas (DLBCLs) and Burkitt lymphomas (BLs) that were positive for Epstein-Barr virus (EBV) infection, indicating that LSH is linked to EBV infection in DLBCL and BL. Interestingly, LSH was mainly localized in the germinal centers of lymph nodes whereas 5-hmC staining localized to areas surrounding the germinal centers.
Conclusions: These findings indicate a critical role for LSH as a biomarker and therapeutic target in follicular germinal center B-cell lymphoma.