Experimental study in vivo ablation of swine pancreas using high-intensity focused ultrasound
Guanghui Huang1, Xin Ye1, Xia Yang1, Aimin Zheng1, Wenhong Li1, Jiao Wang1, Xiaoying Han1, Zhigang Wei1, Min Meng1, Yang Ni1, Chengkun Qin2, Xingbo Fei3, Liulin Xiong4
1 Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China
2 Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China
3 Laboratory of Acoustics, Beijing Yuande Bio-medical Engineering Co., Ltd., Beijing, China
4 Department of Urology, People's Hospital, Beijing University, Beijing, China
Prof. Xin Ye
Department of Oncology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwuweiqi Road, Jinan, Shandong Province 250021
Source of Support: None, Conflict of Interest: None
Objective: The objective of this study is to evaluate the feasibility and safety of high-intensity focused ultrasound (HIFU) for ablation of swine pancreas and to detect the pathological variations in pancreas damage.
Materials and Methods: (a) Eight swine were involved and randomly divided into two groups (Group A and B). HIFU was applied on swine in both groups for in vivo ablation of pancreas. The animals were anesthetized, and the artificial acoustic window was built. Then, the irradiation of FEP-BY02 type HIFU on pancreas was applied. (b) Swine in Group A were euthanized immediately after treating with HIFU to examine variations in pancreas. The biochemical evidence of pancreatitis was evaluated by blood samples collected from swine in Group B before and after HIFU. Then, the pancreas of swine in Group B was euthanized on day 5 after treatment to examine the pancreas. All specimens were visually inspected for both ultrasonic focal damage region (UFDR) and pathological routine by a skilled pathologist.
Results: (a) The vital signs of all animals were stable during HIFU treatment and recovered well after treatment. (b) UFDR were observed in all HIFU irradiation region of the specimens, without significant size difference between the two groups. The coagulation nucleus pyknosis, cytochylema vacuolation, and nucleus membrane disruption were observed after HIFU in both groups. Membranous structure dissolution and inflammatory cell infiltration were also found after HIFU in swine of Group B. (c) There was no significant difference in the levels of blood amylase in swine of Group B before and after HIFU treatment.
Conclusions: It was feasible and safe to use HIFU for ablation of the pancreas in swine.