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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 4  |  Page : 1082-1087

Effects of ameloblastoma-associated fibroblasts on the proliferation and invasion of tumor cells


Faculty of Dentistry, Thammasat University, Khlong Luang, Pathum Thani, Thailand

Correspondence Address:
Yosananda Chantravekin
Faculty of Dentistry, Thammasat University, Rangsit Campus, 99 Moo 18, Paholyothin Rd. T.Khlong Nueng, A.Khlong Luang, Pathum Thani, 12121
Thailand
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.138005

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Context: Ameloblastoma is the most common odontogenic tumor, however, the molecular pathology, especially the role of the tumor stroma, is still unclear. Aims: To investigate the effects of the ameloblatoma-associated fibroblast (AAFs) on the proliferation and invasion of tumor cells. Settings and Design: Cell culture, ELISA, proliferation and invasion assays. Methods and Material: The cocultivations and three-dimensional organotypic cultures of the AAFs and the tumor cells were performed. The gingival fibroblasts (GFs) were used as the control. The levels of TGF-β and HGF in the conditioned media were analyzed using ELISA technique. The MTT proliferation assays and Boyden chamber chemoinvasion assays were also performed. Statistical Analysis Used: ANOVA. Results: Both AAFs and GFs stimulated tumor cell growth. The TGF-β level in the AAF group was more than those of GF group, whereas the HGF levels were not different. The AAF conditioned media also stimulated tumor cell proliferation and invasion more than the GF conditioned media. However, no difference in the thickness and morphology between the AAF and GF groups could be demonstrated in the organotypic models. Conclusions: Both AAFs and GFs support the proliferation of the tumor cells in cocultivation experiment and three-dimensional organotypic cultures. However, the AAFs have a tendency to stimulate the proliferation and induce the invasion more than GFs. Increased TGF-β levels in the AAF condition media suggested the possible role of this growth factor in the ameloblastoma biology.

Abstract in Chinese

釉母细胞瘤相关的纤维母细胞对肿瘤细胞的浸润和增殖的影响 摘要 背景:釉母细胞瘤是最常见的牙源性肿瘤,然而,其分子病理学,尤其是肿瘤间质的作用,目前还不清楚。 目的:探讨了釉母细胞瘤相关的纤维母细胞(AAFS)对肿瘤细胞的浸润和增殖的影响。 设置和设计:采用细胞培养,酶联免疫吸附测定,细胞增殖和侵袭实验。 材料和方法:对AAFs和肿瘤细胞进行协同培养和三维器官型培养。牙龈成纤维细胞(GFS)作为对照。采用ELISA技术分析条件培养基的TGF-β和HGF水平。MTT增殖实验和Boyden小室趋化侵袭实验也被采用。 统计分析:方差分析。 结果:AAFs 和 GFs均刺激肿瘤细胞的生长。TGF-β水平在AAF组高于GF组,而HGF水平是一样的。AAF条件培养基刺激肿瘤细胞增殖和侵袭也超过GF条件培养基。然而,在器官模型上,AAF和GF组之间的厚度和形态无差异。 结论:AAFs 和 GFs支持协同培养实验和三维器官培养实验的肿瘤细胞的增殖。然而,AAFs有刺激增殖和诱导侵袭超过GFS的倾向。在AAF条件培养基中增加的TGF-β水平说明这种生长因子在釉母细胞瘤的生物学方面可能的作用。 关键词:釉母细胞瘤,细胞增殖,细胞生长因子,肿瘤,侵袭性,转化生长因子β



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