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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 4  |  Page : 1057-1062

A survey on anticancer effects of artemisinin, iron, miconazole, and butyric acid on 5637 (bladder cancer) and 4T1 (Breast cancer) cell lines


1 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

Correspondence Address:
Payman Zare
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz
Iran
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Source of Support: University of Tabriz, Faculty of Veterinary Medicine, Conflict of Interest: None


DOI: 10.4103/0973-1482.137975

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Context: Anticancer properties of artemisinin and its derivatives have been shown in many experiments. Aims: Addition of butyric acid, miconazole, and iron to this traditional drug has been done in order to enhance its anticancer potency. Materials and Methods: Cell lines 5637 and 4T1, were cultivated and classified into 13 groups of three each. Different doses of artemisinin with constant doses of iron, miconazole and butyric acid, were added to the cultures. At the end of exposure pathological and enzymatic studies were performed. Results: In four groups treated with different doses of artemisinin and iron, dose-dependent changes were observed. These changes included apoptosis and necrosis with dominance of apoptosis. The supernatant lactate dehydrogenase (LDH) level was increased in a dose-dependent manner, but there was no significant increase in the cell fraction of malonyldialdehyde (MDA) or LDH. In four other groups, which received miconazole, butyric acid and iron in addition to different doses of artemisinin, necrosis was more prominent than apoptosis, and the MDA level did not show any significant change, but LDH was increased. The groups treated with miconazole showed identical changes, with less severity compared to combination therapy groups. In butyric acid-treated groups, the only detectable changes were, mild cell swelling, few apoptosis, and rare necrosis. Conclusions: A combination therapy with artemisinin can be more effective against cancer cells than monotherapy with that. Butyric acid was not effective on cancer cells. Miconazole deviated the nature of cell death from apoptosis to necrosis and it must be used under caution.

Abstract in Chinese

青蒿素、铁、咪康唑和丁酸对5637(膀胱癌)和4T1(乳腺癌)细胞株抗癌作用的调查 摘要 背景:青蒿素及其衍生物的抗癌特性,已在许多实验中被证明。 目的:将丁酸、咪康唑和铁加入到(青蒿素)这一传统药物,以提高其抗癌效力。 材料与方法:将5637和4T1细胞株进行培养,分为13组,每组3株。不同剂量青蒿素加上恒定剂量的铁、咪康唑和丁酸,被加入到培养基。在暴露结束时进行病理和酶的研究。 结果:四组用不同剂量的青蒿素和铁处理,观察剂量依赖性变化,这些变化还包括细胞凋亡和坏死。上清液中乳酸脱氢酶(LDH)水平呈剂量依赖性增加,但细胞碎片中丙二醛(MDA)或LDH没有显著增加。在其他四组,接受咪康唑、丁酸,加入不同剂量铁的青蒿素,见细胞坏死较凋亡更为显著,而MDA水平没有明显改变,但LDH升高。咪康唑处理组表现出相同的变化,但比联合治疗组的严重程度小。丁酸处理组,检测到的变化只有轻度细胞肿胀,很少的细胞凋亡和难得的坏死。 结论:青蒿素联合疗法可以比单独用更有效的对抗癌细胞。丁酸对癌细胞不是很有效。咪康唑可使天然的细胞由凋亡到坏死而死亡,应谨慎使用。 关键词:4T1细胞株,5637细胞株,青蒿素,丁酸,咪康唑



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