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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 4  |  Page : 1045-1051

In vitro anticancer effects of insect tea in TCA8113 cells


Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, China

Correspondence Address:
Xin Zhao
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.137979

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Background: Insect tea is widely used a traditional drink or traditional Chinese medicine in China. This study was conducted with an aim to determine the in vitro anticancer effect of Insect tea in cancer cells. Materials and Methods: The anticancer effects of Insect tea were evaluated in human tongue carcinoma TCA8113 cells using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry analysis, nuclear staining with 4,6-diamidino-2-phenylindole (DAPI), reverse transcription-polymerase chain reaction (RT-PCR) analysis, and western bolt assay. Results: At 200 μg/mL, Insect tea inhibited the growth of TCA8113 cells by 80.7%, which was higher than the inhibition caused by 100 μg/mL Insect tea but lower than that of 200 μg/mL green tea. Compared to the control cancer cells, Insect tea significantly (P < 0.05) induced apoptosis as determined by DAPI staining and flow cytometry analysis results. Insect tea significantly induced apoptosis in cancer cells by upregulating BAX, CASP3, CASP9 and downregulating BCL2. Genes encoding nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were significantly downregulated by Insect tea, demonstrating its anti-inflammatory properties. Insect tea also exerted a great anti-metastasis effect on cancer cells as demonstrated by decreased expression of matrix metalloproteinase (MMP) genes and increased expression of tissue inhibitors of metalloproteinases (TIMPs). Conclusion: The results showed that Insect tea has good in vitro anticancer effects in TCA8113 cells, like green tea.

Abstract in Chinese

虫茶在Tca8113细胞的体外抗癌作用 摘要 背景:虫茶在中国是广泛应用的一种传统饮料或中药。本研究旨在确定虫茶对癌细胞的体外抗癌效果。 材料和方法:对虫茶对人舌癌Tca8113细胞的抗癌作用进行评价,采用 3-(4,5-二甲基-2-噻唑)-2,5-苯基溴化(MTT)测定,流式细胞仪分析,核染色与4,6-二脒基-2-苯基吲哚(DAPI),逆转录聚合酶链反应(RT PCR)分析,和免疫印迹试验。 结果:200 μg/mL浓度的虫茶对TCA8113细胞的生长增殖的抑制率为80.7%,高于100μg/mL虫茶但低于200μg/mL绿茶。DAPI染色和流式细胞仪分析结果显示,相比于对照癌细胞,虫茶显著诱导细胞凋亡(P<0.05)。虫茶显著诱导肿瘤细胞凋亡可能通过上调Bax,CASP3,CASP9和下调Bcl-2。活化B细胞的基因编码核转录因子-κ轻链增强子(NF-B),诱导型一氧化氮合酶(iNOS),和环氧化酶2(COX 2)显著下调,表明虫茶抗炎的特性。虫茶也产生了巨大的抗转移作用,表现在癌细胞的基质金属蛋白酶(MMP)的表达下降和基质金属蛋白酶组织抑制剂(TIMPs)表达增加。 结论:结果表明,虫茶和绿茶一样,在Tca8113细胞的体外抗癌效果好。 关键词:抗癌,绿茶,人舌癌Tca8113细胞,虫茶,中药



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