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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 4  |  Page : 1040-1044

Effect of melatonin on antioxidant status and circadian activity rhythm during hepatocarcinogenesis in mice


1 Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India
2 University of Malaya Centre for Proteomics Research; Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

Correspondence Address:
Perumal Subramanian
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.138227

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Aim: Alteration of circadian systems can cause cancer and affects its development and response to therapeutics. The present study investigates whether cancer can disrupt circadian locomotor rhythms and evaluated the influence of melatonin (MLT) and oxaliplatin on the levels of antioxidants and circadian locomotor activity rhythms in N-nitrosodiethylamine (NDEA)-induced liver tumor in Indian field mouse (Mus booduga). Materials and Methods: Effects of NDEA, NDEA, and MLT, as well as NDEA and oxaliplatin, on levels of mice liver marker enzymes and antioxidants and their circadian locomotor activity rhythm were assessed. Results: Treatment of mice with NDEA caused significant alteration of their liver marker enzymes [aspartate transaminase and alanine transaminase; P< 0.05 Duncan's multiple range test (DMRT) test] antioxidant levels (superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase; P< 0.05, DMRT test] and circadian locomotor activity rhythm, which were abrogated when the animals were also given MLT or the anticancer drug, oxaliplatin. Conclusion: Our study demonstrated that the circadian clock was disturbed by hepatocarcinogenesis and the effects could be reversed by the chronobiotic, MLT.

Abstract in Chinese

褪黑素在肝癌小鼠中对抗氧化状态和昼夜活动节律的影响 摘要 目的:昼夜系统变化可以导致癌症并影响其发展和对治疗的反应。本研究基于二乙基亚硝胺(NDEA)诱导的肝肿瘤印度田鼠模型,探讨癌症可以扰乱昼夜活动节律,评估褪黑激素(MLT)和奥沙利铂对抗氧化剂和昼夜活动节律的影响。 材料和方法:对NDEA、NDEA联合褪黑素,以及NDEA联合奥沙利铂对于小鼠肝标记酶和抗氧化剂及它们的昼夜自发活动节律水平的影响进行评估。 结果: 经NDEA处理的小鼠导致它们的肝标记酶[天冬氨酸转氨酶和丙氨酸转氨酶;P<0.05邓肯多范围测试(DMRT)试验]显著改变,抗氧化剂水平(超氧化物歧化酶,过氧化氢酶,谷胱甘肽过氧化物酶,谷胱甘肽S转移酶;P<0.05,DMRT试验)及自发昼夜活动节律改变,直到给予小鼠MLT或抗癌药物奥沙利铂时(这些变化)才终止。 结论:我们的研究表明,生物钟可由肝癌干扰,其影响可以由生物时相药MLT回复。 关键词:抗氧化酶,昼夜节律,肝癌,褪黑激素,MLT,NDEAN,亚硝胺



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