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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 4  |  Page : 1004-1007

The comparison of antimutagenicity and anticancer activities of Echinophora platyloba DC on acute promyelocytic leukemia cancer cells


1 Department of herbal medicine, Institue for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran
2 Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran, Iran

Correspondence Address:
Mehrdad Hashemi
Department of Genetics, Islamic Azad University, Tehran Medical Branch, Tehran
Iran
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Source of Support: Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran, Conflict of Interest: None


DOI: 10.4103/0973-1482.137907

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Background: Cancer is one of the main causes of mortality in the world which is created by the effect of enviromental physico-chemical mutagen and carcinogen agents. In the last years, many studies have been performed on the anticancer effects of flavonoids. Echinophora platyloba DC plant (Khousharizeh) is one of the indigenous medicinal plants which is used as a food seasoning and medicine in Iran. Materials and Methods: The extract was evaluated in terms of antimutagenicity properties by a standard reverse mutation assay (Ames Test). This was performed with histidine auxotroph strain of Salmonella typhimurium (TA100). Thus, it requires histidine from a foreign supply to ensure its growth. The afore mentioned strain gives rise to reverted colonies when expose to carcinogen substance (Sodium Azide). The other objective of this study was to examine the in vitro cytotoxic activity of cell death of crude methanolic extracts prepared from Echinophora platyloba on Acute Promyelocytic Leukemia cell line (NB4). Cytotoxicity and viability of methanolic extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dye exclusion assay. Results: In Ames test the extract prevented the reverted mutations and the hindrance percent was 93.4% in antimutagenicity test. Data obtained from this assay indicated that the extract significantly reduced the viability of NB4 cells and inhibited cell growth in a dose dependent manner. Conclusion: This study demonstrates the antimutagenicity effect of Echinophora Platyloba and suggests that it has a potential as an anticancer agent.

Abstract in Chinese

Echinophora platyloba DC对急性早幼粒细胞白血病癌细胞的抗突变、抗癌活性的比较 摘要 背景:癌症是世界上主要致死的原因之一,其发生归因于环境的物理化学诱变剂和致癌物介质。在过去的几年中,许多研究已经对黄酮类化合物的抗癌作用进行了(研究)。Echinophora platyloba DC植物(khousharizeh)是一种本土药用植物,在伊朗它被用作调味食品和药品。 材料和方法:通过一个标准的反向突变试验(Ames试验)来评价该提取物的抗突变特性。通过鼠伤寒沙门氏菌组氨酸缺陷型菌株(TA100)进行。因此,它需要从外界供应组氨酸以确保其成长。当上述菌株暴露于致癌物质(叠氮化钠)时,即回复成原来的克隆株。该研究的另一目的是检查echinophora platyloba的粗甲醇提取物对急性早幼粒细胞白血病细胞株(NB4)在体外细胞死亡的细胞毒活性。用3-(4,5二甲基噻唑-2-基)-2,5-联苯四唑溴化物(MTT)和染料排斥试验对甲醇提取物的细胞毒性和活性进行了评估。 结果:Ames试验中提取防止突变修复,在抗突变试验中阻碍率为93.4%。从这个试验中获得的数据表明,提取物能显著降低NB4细胞活性,以剂量依赖的方式抑制细胞生长。 结论:本研究证明platyloba echinophora的抗诱变效果,它具有作为抗癌剂的潜力。 关键词:急性早幼粒细胞白血病,Ames试验,echinophora platyloba DC,NB4细胞,MTTY试验



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