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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 2  |  Page : 317-323

Analysis of the chromosomal aneuploidy by interphase fluorescence in situ hybridization (FISH) in Squamous cell carcinoma of the cervix in Jammu region of J and K state


1 Institute of Human Genetics, University of Jammu, Jammu and Kashmir, India
2 Department of Gynecology and Obstetrics, SMGS Hospital/Govt. Medical College, Jammu, India
3 Human Genetic Research Cum Counseling Centre, Govt. Medical College/University of Jammu, Jammu, India

Correspondence Address:
Subash Gupta
Centre Coordinator, HGRCC, GMC/JU, Jammu
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.136612

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Aim: The present study was aimed to analyze the chromosomal changes obtained by using FISH. Fluorescence in situ hybridization (FISH) is a powerful technique used in the detection of chromosomal abnormalities. Materials and Methods: During the present study, FISH technique was carried out in 26 cases of Squamous cell carcinoma of the cervix using Centromere Enumeration Probes, CEP for chromosome 3 and 17. Statistical tests including ANOVA and Chi square tests were used to analyze the numerical chromosome aberration data obtained by FISH analysis. FISH technique was also carried out in 10 healthy samples (control) by using both CEP 3 and CEP 17 probe. Results and Discussion: FISH results of CEP 3 in the present study showed the frequent gains of chromosome 3 in Squamous cell carcinoma over the chromosome 17. ANOVA results were found to be significant for both CEP 3 and CEP-17 in Squamous cell carcinoma of the cervix. Chi square test (disease v/s control) was also used to study the significant difference between the patients and the controls and it was found to be significant for both chromosomes 3 and 17.

Abstract in Chinese

荧光原位杂交(FISH)技术检测J和K州Jammu地区的宫颈鳞状细胞癌异倍体染色体 摘要 目的:荧光原位杂交(FISH)是用于染色体异常的检测一个强大的技术,本研究拟利用FISH技术分析染色体的改变。 材料与方法: FISH技术分析26例宫颈鳞状细胞癌患者和10例健康对照3号和17号染色体(CEP),数据用统计学分析,包括方差分析和卡方检验。 结果与讨论:在目前的研究FISH结果表明FISH结果在鳞状细胞癌中3号染色体的检测频度于17号染色体。方差分析结果表明在宫颈鳞癌中CEP 3和CEP 17都有重要意义。卡方检验(病人与对照)也表明患者和对照组之间差异显著,无论是染色体3号还是17号。 关键词:癌,宫颈,染色体,荧光原位杂交



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