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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 89-96

Proteomic analysis reveals novel proteins associated with progression and differentiation of colorectal carcinoma


Department of General Surgery, The Third Affiliated XiangYa Hospital of Central South University,Changsha, Hunan 410013, China

Correspondence Address:
Xiaorong Li
Department of General Surgery, The Third Affiliated XiangYa Hospital of Central South University, No. 138 Tongzipo Road, Yuelu District, Changsha, Hunan 410013
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.131396

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Aim: The objective of this study is to characterize differential proteomic expression among well-differentiation and poor-differentiation colorectal carcinoma tissues and normal mucous epithelium. Materials and Methods: The study is based on quantitative 2-dimensional gel electrophoresis and analyzed by PDquest. Results: Excluding redundancies due to proteolysis and posttranslational modified isoforms of over 600 protein spots, 11 proteins were revealed as regulated with statistical variance being within the 95 th confidence level and were identified by peptide mass fingerprinting in matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Progression-associated proteins belong to the functional complexes of tumorigenesis, proliferation, differentiation, metabolism, and the regulation of major histocompatibility complex processing and other functions. Partial but significant overlap was revealed with previous proteomics and transcriptomics studies in CRC. Among various differentiation stage of CRC tissues, we identified calreticulin precursor, MHC class I antigen (human leukocyte antigen A ), glutathione S-transferase pi1, keratin 8, heat shock protein 27, tubulin beta chain, triosephosphate, fatty acid-binding protein, hemoglobin (deoxy) mutant with val b 1 replaced by met (HBB), and zinc finger protein 312 (FEZF2). Conclusions: Their functional networks were analyzed by Ingenuity systems Ingenuity Pathways Analysis and revealed the potential roles as novel biomarkers for progression in various differentiation stages of CRC.

Abstract in Chinese

蛋白生物学研究分析揭示结直肠癌进展和分化的新蛋白 目的:本研究的目标是描述分化好的和分化差的结直肠癌组织及正常黏液上皮细胞的不同蛋白质生物学表达的特征。 材料和方法:本试验是基于定量的二维凝胶电泳法并通过渗透试验分析。 结果:排除过多的由蛋白质水解和转译修饰后的亚型的600多蛋白质斑点,11种蛋白质具有统计学差异(95%可信区间),通过肽指纹基质辅助激光解吸/电离飞行时间质谱学辩认出。进展相关的蛋白属于功能复杂的肿瘤发生、增殖、分化、新陈代谢及重大组织适应整合和其它功能。先前结直肠癌的蛋白质组学和转录物组学研究揭示了部分但是有重要意义的重叠。在结直肠癌组织的不同分化阶段,我们找到了钙网织蛋白前体,MHC I类抗原(人白细胞抗原A),谷胱甘肽-S-转移酶pi1,角蛋白8,热激蛋白27,微管蛋白β链,磷酸丙糖,脂肪酸结合蛋白,血红蛋白(脱氧)变异体(val b1被met取代)(HBB),及锌指蛋白312(FEZF2)。 结论:它们的功能网通过精巧的系统、精巧的通路分析,揭示了结直肠癌的进展和分化中蛋白质角色作为一个新的生物印迹。 关键词:结直肠癌,分化,质谱学,蛋白质组,二维凝胶电泳法



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