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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 84-88

Validation of a high-performance liquid chromatographic ultraviolet detection method for the quantification of vandetanib in rat plasma and its application to pharmacokinetic studies


1 State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China
2 Department of Oncology, Chengdu Fifth People's Hospital, Chengdu 611130, China

Correspondence Address:
Yinglan Zhao
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.131393

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Aim: To develop a simple and sensitive high-performance liquid chromatography (HPLC) assay with ultraviolet detection method of vandetanib in rat plasma. Materials and Methods: Samples were extracted with methanol and acetonitrile, evaporated, and then the residue was reconstituted in mobile phase. Vandetanib and the internal standard (I.S.) trazodone hydrochloride were separated with gradient elution (on a C18 Atlantis® column using a mobile phase of acetonitrile/0.5% triethylamine, pH 3.0, with a flow rate of 1.0 ml/min), then detected at 341 nm. Results: A linear curve over the concentration range of 80-4000 ng/ml (R 2 = 0.9998) was obtained. Intra- and inter-assay accuracy ranged from 98.80% to 103.08% and 95.32% to 98.40%, with high precision (R.S.D. % <5%), respectively. The mean absolute recovery was 96.65%. Conclusion: A simple and sensitive HPLC assay with ultraviolet detection method was developed for the determination of vandetanib in rat plasma. This method is sufficient for pharmacokinetic studies of vandetanib in small animals and may be applied to human pharmacokinetic studies.

Abstract in Chinese

高效液相色谱-紫外检测法应用于凡德他尼在大鼠血浆定量测定中的校验及它在药代 动力学研究中的应用 目的:开发一种简单敏感的高效液相色谱法(HPLC)联合紫外检测法应用于凡德他尼在大鼠血浆中的测定。 材料和方法:用甲醇和乙腈提取样本、蒸馏,残留物再构成移动相。凡德他尼和内部标准的曲唑酮氢氯化物被梯度洗提分离(在C18亚特兰蒂斯®列使用乙腈/0.5%三乙胺移动相, pH 3.0,流动率 1.0ml/min),检测到341nm。 结果:80~4000ng/ml(R2=0.9998)浓度范围的线形弧度被观察到。试验内部和试验间的精准度分别为98.80%~103.08%和95.32%~98.40%,精度高(R.S.D.%<5%)。平均绝对回收率为96.65%。 结论:开发了一种简单敏感的HPLC方法联合紫外检测法用来测定大鼠血浆中的凡德他尼。这种方法可用于凡德他尼在小动物及人体中的药代动力学研究。 关键词:高效液相色谱-紫外检测,药代动力学,凡德他尼



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