Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 159-164

Adipose derived stem cells isolated from omentum: A novel source of chemokines for ovarian cancer growth


1 Shiraz Institute for Cancer Research, Departments of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Iran
3 Department of Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Abbas Ghaderi
Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, School of Medicine, Shiraz
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.131451

Rights and Permissions

Background: The main site of ovarian cancer metastasis is the omentum. Omental adipose tissue is known for contribution to the tumor growth and metastasis through different mechanisms. Aims: In the present study, adipose derived stem cells (ASCs) were isolated from the omentum of patients with ovarian cancer and those with ovarian cysts and the expression of chemokines, chemokine receptors and cytokines were analyzed. Materials and Methods: ASCs were isolated from omental adipose tissues obtained of 10 ovarian cancer and 25 ovarian benign cyst patients. Our investigations were done by quantitative real time-polymerase chain reaction, flowcytometry, western blot and also enzyme-linked immunosorbent assay. Result: Expression of CXCL-10 and CCR5 showed statistically significant difference between omentum derived ASCs of ovarian cancer patients compared with those with benign cysts (P < 0.05). Expression of interleukin-10 also detected in the supernatant of cultured malignant ASCs. Conclusion: Omental adipose tissue may play crucial roles for tumor promotion through the expression of tumor promoting chemokines. Accordingly, tumor surrounding adipose tissue may be a novel target for immunotherapy of cancer.

Abstract in Chinese

网膜分离的脂肪干细胞:卵巢癌生长趋化因子的新来源 背景:卵巢癌转移的主要部位是网膜。大网膜脂肪组织在肿瘤的生长和转移上贡献巨大,通过不同的机制。 目的:在本研究中,脂肪干细胞(ASCs)在卵巢癌与卵巢囊肿患者的网膜中分离出来,并对其趋化因子表达,趋化因子受体和细胞因子进行了分析。 材料和方法:从10例卵巢癌和25例卵巢良性囊肿患者的大网膜脂肪组织中分离出脂肪干细胞ASCs。我们的研究,通过实时定量聚合酶链反应,流式细胞仪,蛋白免疫印迹法和酶联免疫吸附测定法。 结果:卵巢癌与良性囊肿患者之间网膜脂肪干细胞CXCL10和CCR5的表达差异有统计学意义(P<0.05)。在培养的恶性脂肪干细胞上清液中也检测到了白细胞介素10的表达。 结论:大网膜脂肪组织可以通过肿瘤促进趋化因子的表达在肿瘤促进上发挥重要的作用。因此,肿瘤周围的脂肪组织可能是肿瘤免疫治疗的新靶点。 关键词:脂肪干细胞,卵巢癌,卵巢囊肿,网膜



[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2228    
    Printed63    
    Emailed1    
    PDF Downloaded154    
    Comments [Add]    

Recommend this journal