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ORIGINAL ARTICLE
Year : 2014  |  Volume : 10  |  Issue : 1  |  Page : 133-141

How prostate-specific membrane antigen level may be correlated with stemness in prostate cancer stem cell-like cell populations?


Research Genetic Cancer Centre Ltd, Megalou Alexandrou 115 Street, 53070, Filotas, Greece

Correspondence Address:
Papasotiriou Ioannis
Megalou Alexandrou 115 Street, 53070, Filotas
Greece
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.131461

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Background: Prostate-specific membrane antigen (PSMA) is a widely used targeted molecule in prostate patients. The present research, attempts to support the hypothesis that PSMA expression in prostate cancer stem cell-like (CSC) cell populations may be correlated with nanog and other transcription factors in different stages of prostate carcinomas. Materials and Methods: To provide more accurate evidence of the above, a population of prostate CSCs was isolated and analyzed using different protocols. The first method was based in the ability of CSCs to form spherical colonies in semi-suspension of a culture. A qPCRbased protocol and a flow cytometric analysis protocol were chosen to test the presence of stemness markers and PSMA in the selected populations. Results: The formation of micro-sphere in semi-suspension has been pointed out. In the other panels of the test, the linear correlation between PSMA and nanog in gene and protein level was shown. However, the statistical analysis including the coefficient of variationand standard deviation's values) has proved that there were differences in PSMA expression between cancer cells and CSCs. Conclusion: The previous analysis has pointed out that PSMA expression may be correlated with nanog's expression as well as with other confounders in a population of prostate CSCs.

Abstract in Chinese

前列腺特异性膜抗原水平可能与前列腺癌干细胞样细胞群的干细胞特性相关? 背景:在前列腺癌患者中前列腺特异性膜抗原(PSMA)是一种广泛使用的靶向分子。目前的研究,试图支持这一假设,在前列腺癌干细胞样(CSC)细胞群中PSMA表达可能与前列腺癌的不同阶段中Nanog(一种基因)和其他转录因子相关。 材料和方法:为提供上述更准确的依据,使用不同的方法分离和分析一群前列腺干细胞样细胞群。第一种方法是基于CSCs在半悬浮环境中形成球形聚集的能力。基于AqPCR的方法和流式细胞仪分析方法用来检测在选定的细胞群中干细胞标志物和PSMA的存在。 结果:已明确半悬浮环境中微球细胞群的形成,其他测试也显示PSMA和Nanog基因蛋白水平呈线性相关。然而,统计分析包括的标准差值的变异系数已经证明,在肿瘤细胞和干细胞之间的PSMA表达有差异。 结论:PSMA的表达可能与Nanog的表达以及前列腺癌干细胞群的其他混杂因素有关。 关键词:癌干细胞样细胞,前列腺癌,前列腺特异性膜抗原,干细胞特性



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