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ORIGINAL ARTICLE
Year : 2013  |  Volume : 9  |  Issue : 1  |  Page : 54-59

M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway


Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, National Research Center for Genitourinary Oncology, Beijing, China

Correspondence Address:
Wujiang Liu
Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, National Research Center for Genitourinary Oncology, Beijing
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.110367

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Objectives: To investigate the effect of M-ds-P21 on the apoptosis of bladder cancer T24 cells and its potential mechanism. Materials and Methods: Effect of M-ds-P21 on T24 cells were assessed by cell morphology and Western blot. Apoptosis was quantified by Annexin-V flow-cytometry analysis. To uncover the role of P53 in M-ds-P21-mediated apoptosis of T24 cells, we knocked down P53 before treating cells with M-ds-P21, and then assayed P21 and apoptosis-related protein by Western blot. To uncover the mechanism by which M-ds-P21 played stronger effect than ds-P21, we performed confocal microscope analyses. Results: Both M-ds-P21 and ds-P21 treatment changed the cell morphology, leading to cell apoptosis after 3 days. Apoptosis induced by M-ds-P21 and ds-P21 treatment is not P53-dependent but caspase-dependent. Compared with ds-P21, M-ds-P21 significantly increased the bioavailability of ds-RNA in T24 cells. Conclusions: M-ds-P21 treatment induces more apoptotic population than ds-P21 does. The mechanism for stronger effect of M-ds-P21 is partly due to the enhanced bioavailability of ds-RNA in human bladder cancer T24 cells, and not P53-dependent but caspase-dependent.


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