A preliminary study on the radiation-resistance mechanism in ovarian cancer
Qi Liao1, Hong-mei Zhang2, Hai-hua Li3, Ren Zhou3, Hui-ling Mao3, Yu-bing Chen4, Man-Hua Cui5
1 Department of Gynaecology and Obstetrics, Second Teaching Hospital of Jilin University,Changchun; Department of Gynaecology and Obstetrics, The Da Qing Long Nan Hospital, Daqing, HeiLong Jing, China
2 Laboratory of TANG Ao-qing Distinguished Professor, China-Japan Union Hospital of Jilin University, Changchun, P. R, China
3 Department of Gynaecology and Obstetrics, The Da Qing Long Nan Hospital, Daqing, HeiLong Jing, China
4 Department of Radiotherapy, Second Teaching Hospital of Jilin University, Changchun, China
5 Department of Gynaecology and Obstetrics, Second Teaching Hospital of Jilin University, Changchun, China
Second Clinical Hospital of Jilin University, 218 Ziqiang street, Changchun 130041, P. R
Source of Support: None, Conflict of Interest: None
Aim: The present study was designed to explore the radiation-resistance mechanism by interfering in checkpoints kinase 1 (CHK1) and DNA-activated protein kinase (DNA-PK) genes with short hairpin RNA (shRNA) transfection into Skov3 cells derived from ovarian cancer and HeLa cells derived from cervical cancer.
Materials and Methods: The cultured Skov3 and HeLa cells were transfected with plasmid vectors containing CHK1 shRNA and DNA-PK shRNA, respectively, through Lipofectimine™ 2000 mediation, and cultured for 20 hours before exposure to 2 Gy X-radiation. The cells were harvested 4 and 28 after X-irradiation respectively then washed 3 times with PBS. These cells were stained with Annexin V/PI and applied by flow cytometer to analyze alteration of apoptosis with software CellQuest.
Results: The apoptotic response in Skov3 cells to X-radiation was significantly lower than that in HeLa cells at 4 hour (t = 15.22, P < 0.001) and 28 hours (t = 15.78, P < 0.001) of post-irradiation. The shRNA might not affect the apoptosis of Skov3 and HeLa cells, while shRNA-transfection significantly enhanced the apoptotic response in Skov3 cells to X-radiation as compared with that in HeLa cells.
Conclusions: The present work suggests that the CHK1 and DNA-PK genes are very likely to play a role in developing a radiation resistance in ovarian cancer.