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ORIGINAL ARTICLE
Year : 2012  |  Volume : 8  |  Issue : 2  |  Page : 226-231

Downregulation of gene MDR1 by shRNA to reverse multidrug-resistance of ovarian cancer A2780 cells


1 Department of Pathogenic Biology and Immunology, Zhongda Hospital, Medical School, Southeast University, Nanjing, 210009, China
2 Department of Gynecology and Obstetrics, Zhongda Hospital, Medical School, Southeast University, Nanjing, 210009, China

Correspondence Address:
Jun Dou
Department of Pathogenic Biology and Immunology, Medical School, Southeast University, Nanjing 210009
China
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Source of Support: Supported in part by the National Natural Science Foundation of China (No. 81071769), and in part by the Groundbreaking experimental design of undergraduate student in China (No. G2007059), and in part by the 973 Program of China (No.2011CB933500), Conflict of Interest: None


DOI: 10.4103/0973-1482.98975

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Background: To explore the effects of downregulated multidrug-resistance P-glycoprotein (MDR1/ABCB1) and reversed multidrug-resistance in human A2780 ovarian cancer cells. Materials and Methods: Three shRNAs targeting the MDR1 gene were synthesized, and cloned into plasmid pSUPER-enhanced green fluorescent protein 1 (EGFP1). The formed pSUPER-EGFP1-MDR1-shRNAs were transfected into the A2780 cells, respectively, and the quantitative reverse transcription polymerase chain reaction and western blot were used respectively to determine the MDR1 expression. The multidrug-resistance (MDR) of the MDR1-shRNAs transfected A2780 cells to chemotherapy drugs in vitro and in tumor-bearing nude mice were respectively evaluated. Results: The MDR1 shRNA expression resulted in decreased P-glycoprotein expression in the transfected A2780 cells. The MDR1-shRNA2 transfected cells showed that the sensitivities to chemotherapy drugs were higher than other shRNAs transfected A2780 cells, and that the formed tumor in mice grew slower than those of other mice after paclitaxel was injected into tumor-bearing nude mice. Conclusions: Our data demonstrates that the RNA interference could knock down gene MDR1 and reduce the P-glycoprotein expression, and partly reverse the MDR of A2780 cells in vitro and in vivo. These results suggest that MDR-1 is an effective therapeutic target for human ovarian caner treatment.


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