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ORIGINAL ARTICLE
Year : 2011  |  Volume : 7  |  Issue : 4  |  Page : 427-432

Expression of fascin protein and mRNA in the KB carcinoma cell line following treatment with doxorubicin


1 Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran
2 Molecular Research Lab, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3 Molecular Research Lab, Department of Pharmacology and Toxicology, Faculty of Pharmacy; Department of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran

Correspondence Address:
Shahroo Etemad-Moghadam
Dental Research Center, Tehran University of Medical Sciences, Ghods St., Enghelab Ave., P.O. Box: 14155-5583, Postal code: 14174 Tehran
Iran
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Source of Support: The present study was supported by Iran National Science Foundation, INSF, Conflict of Interest: None


DOI: 10.4103/0973-1482.92009

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Context: Fascin is an actin-bundling protein that has been shown to be overexpressed in various carcinomas, but the effect of doxorubicin on its protein and mRNA has not been investigated. Aims: The aim of the present study is to evaluate any changes that may be induced by doxorubicin on the fascin molecule in a cancer cell line. Settings and Design: Experimental study. Materials and Methods: The expression of fascin protein and mRNA in the human KB carcinoma cell line was evaluated by qRT-PCR, and immunocytochemistry following treatment with doxorubicin. Apoptosis was also analyzed using flow cytometry. Statistical Analysis Used: Group comparisons were performed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test to determine differences between groups (P < 0.05). Results: Treatment with doxorubicin promoted a significant increase of fascin mRNA and decrease of fascin protein (P <0.05). Doxorubicin also induced apoptosis in the KB cell line. Conclusions: Regarding the changes in the actin-bundling protein, fascin, which was found following treatment with doxorubicin, it can be hypothesized that this drug may possess antimigratory characteristics by influencing fascin. Further studies are suggested to clarify this theory.


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