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ORIGINAL ARTICLE
Year : 2011  |  Volume : 7  |  Issue : 3  |  Page : 314-324

In vitro and in vivo targeted delivery of photosensitizers to the tumor cells for enhanced photodynamic effects


1 Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig S K Mazumdar Road, Delhi, India; Department of Radiation Oncology, and Sylvester Comprehensive Cancer Center, University of Miami, Miami, FL 33136, USA
2 Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig S K Mazumdar Road, Delhi, India
3 Division of Cyclotron and Radiopharma-ceuticals, Institute of Nuclear Medicine and Allied Sciences, Brig S K Mazumdar Road, Delhi, India
4 Department of Zoology, University of Delhi, Delhi, India
5 Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig S K Mazumdar Road, Delhi, India; Eco-Development Foundation, New Delhi, India

Correspondence Address:
Seema Gupta
Department of Radiation Oncology, Sylvester Comprehensive Cancer Center, University of Miami, 1550 Northwest 10th Avenue, PAP Building, Room 117, Miami, FL 33136, USA

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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1482.87035

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Background: Efficacy of photodynamic therapy can be enhanced by improving uptake, localization, and sub-cellular localization of sensitizers at the sensitive targets. Materials and Methods: Uptake, localization, and photodynamic effects of hematoporphyrin derivative (HpD, Photosan-3; PS-3) and disulfonated aluminum phthalocyanine (AlPcS 2 ) were studied either encapsulated in liposomes or conjugated to a monoclonal antibody to carcinoembryonic antigen (anti-CEA) in a brain glioma cell line, BMG-1. Results: Although the total uptake with encapsulated or conjugated sensitizers was less than the free sensitizers, photodynamic efficiency was higher due to the localization of the sensitizer at the sensitive targets. Biodistribution of intravenously administered technetium ( 99m Tc)-labeled PS-3 analyzed by gamma camera imaging showed maximum accumulation in the liver followed by tumor. Tumor/muscle (T/N) ratio of free PS-3 was higher compared to encapsulated or conjugated PS-3 but the accumulation of PS-3 significantly reduced in brain and cutaneous tissue following modulated delivery. Pharmacokinetics suggested faster accumulation of encapsulated and conjugated PS-3 in the tumor. Conclusion: Localization of sensitizers at sensitive targets and reduced accumulation in normal tissues with liposome encapsulation and antibody conjugation suggest that these two delivery systems can potentially enhance the efficacy of photodynamic treatment.


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